Metabolomics emphasizes the full complement of plant metabolites between genetics and phenotype. The session will address tools and strategies for metabolite profiling, such as chromatography, mass spectrometry, and NMR spectroscopy. Speakers will address primary metabolites like sugars, amino acids, and lipids, as well as secondary metabolites for defense and signaling. Case studies will demonstrate how metabolomic information sheds light on plant reactions to drought, salinity, and pathogens. Emphasis will be placed on integrating with genomics and transcriptomics in constructing complete metabolic networks. The presentation will also feature metabolomics in plant breeding for enhanced nutritional content and flavor. Through the understanding of metabolic pathways, the audience will gain an appreciation of how metabolomics bridges molecular biology and ecological function and agricultural innovation.