Plant tissue culture is a mainstay of contemporary plant biotechnology. This session emphasizes in vitro culture techniques principles and applications. We will cover micropropagation for mass plant production. Somatic embryogenesis and organogenesis processes will be elaborated. Case studies will exhibit use in horticulture and forestry. Experts will present progress in genetic transformation via tissue culture. . The session includes strategies for the generation of disease-free planting material. Biotechnological developments in genome editing using culture systems will be discussed. We will discuss automation and scale-up of tissue culture operations. The industry applications in agriculture and pharmaceuticals will be discussed. The session will feature success stories in crop improvement. The participants will learn about practical and commercial viewpoints.